abstract：BACKGROUND:Δ(9)-Tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), and cannabinol (CBN) were measured in breath following controlled cannabis smoking to characterize the time course and windows of detection of breath cannabinoids. METHODS:Exhaled breath was collected from chronic (≥4 times per week) and occas...

journal_title：Clinical chemistry

authors： Himes SK,Scheidweiler KB,Beck O,Gorelick DA,Desrosiers NA,Huestis MA

更新日期：2013-12-01 00:00:00

abstract：BACKGROUND:Cystic fibrosis is a life-threatening genetic disorder that has been associated with mutations in the CFTR [cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)] gene. Hundreds of CFTR mutations have been detected to date. Current CFTR genotyping assays target a s...

journal_title：Clinical chemistry

authors： Abou Tayoun AN,Tunkey CD,Pugh TJ,Ross T,Shah M,Lee CC,Harkins TT,Wells WA,Tafe LJ,Amos CI,Tsongalis GJ

更新日期：2013-10-01 00:00:00

abstract：BACKGROUND:The use of nonstandardized N-terminal pro-B-type natriuretic peptide (NT-proBNP) assays can contribute to the misdiagnosis of heart failure (HF). Moreover, there is yet to be established a common consensus regarding the circulating forms of NT-proBNP being used in current assays. We aimed to characterize and...

journal_title：Clinical chemistry

authors： Foo JY,Wan Y,Schulz BL,Kostner K,Atherton J,Cooper-White J,Dimeski G,Punyadeera C

更新日期：2013-10-01 00:00:00

abstract：BACKGROUND:There are 45 known genetic diseases that impair the lysosomal degradation of macromolecules. The loss of a single lysosomal hydrolase leads to the accumulation of its undegraded substrates in tissues and increases of related glycoconjugates in urine, some of which can be detected by screening of free oligosa...

journal_title：Clinical chemistry

authors： Xia B,Asif G,Arthur L,Pervaiz MA,Li X,Liu R,Cummings RD,He M

更新日期：2013-09-01 00:00:00

abstract：BACKGROUND:About 5% of patients with neurofibromatosis type 1 (NF1) bear constitutional microdeletions that encompass NF1 (neurofibromin 1) and neighboring genes. These patients are characterized by the development of a high number of dermal neurofibromas (dNFs), mental retardation, and an increased risk of developing ...

journal_title：Clinical chemistry

authors： Terribas E,Garcia-Linares C,Lázaro C,Serra E

更新日期：2013-06-01 00:00:00

abstract：BACKGROUND:Measurement of serum thyroglobulin (Tg) is used to monitor patients after treatment for differentiated thyroid carcinoma (TC). Difficulty in using Tg as a biomarker of the recurrence of TC in many patients stems from the presence of endogenous anti-Tg autoantibodies (Tg-AAbs), which can interfere with immuno...

journal_title：Clinical chemistry

authors： Kushnir MM,Rockwood AL,Roberts WL,Abraham D,Hoofnagle AN,Meikle AW

更新日期：2013-06-01 00:00:00

abstract：BACKGROUND:5α-Reductase 2 deficiency (5ARD) is a known cause of 46,XY disorders of sex development (DSD). Traditionally, the diagnosis relies on dihydrotestosterone (DHT) measurement, but the results are often equivocal, potentially leading to misdiagnosis. We reviewed alternative approaches for diagnosis of 5ARD. MET...

journal_title：Clinical chemistry

authors： Chan AO,But BW,Lee CY,Lam YY,Ng KL,Tung JY,Kwan EY,Chan YK,Tsui TK,Lam AL,Tse WY,Cheung PT,Shek CC

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND:Plasma concentrations of the peptides midregional proadrenomedullin (MR-proADM), midregional proatrial natriuretic peptide (MR-proANP), and C-terminal endothelin-1 precursor fragment (CT-proET-1) are increased in various cardiovascular conditions. However, there is limited information about the association a...

journal_title：Clinical chemistry

authors： Kollerits B,Sturm G,Lamina C,Hammerer-Lercher A,Rantner B,Stadler M,Ziera T,Struck J,Klein-Weigel P,Fraedrich G,Kronenberg F

更新日期：2013-04-01 00:00:00

abstract：BACKGROUND:Total prostate-specific antigen (tPSA) is flawed for prostate cancer (PCa) detection. [-2]proprostate-specific antigen (p2PSA), a molecular isoform of free PSA (fPSA), shows higher specificity compared with tPSA or percentage of free PSA (%fPSA). The prostate health index (Phi), a measure based on p2PSA and ...

journal_title：Clinical chemistry

authors： Stephan C,Vincendeau S,Houlgatte A,Cammann H,Jung K,Semjonow A

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:Kallikrein-related peptidase 6 (KLK6), a member of the serine protease family of kallikrein (KLK) genes, is dysregulated in ovarian carcinomas (OCa) and its overexpression is associated with poor prognosis. Regulation of its expression is poorly understood and is likely to be influenced by multiple mechanism...

journal_title：Clinical chemistry

authors： Bayani J,Kuzmanov U,Saraon P,Fung WA,Soosaipillai A,Squire JA,Diamandis EP

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:The promise of targeted therapies in molecularly defined subsets of cancer has led to a transformation of the process of drug development in oncology. To target cancer successfully and precisely requires high-quality translational data. Such data can be generated by the use of biomarkers that answer key ques...

journal_title：Clinical chemistry

authors： Modur V,Hailman E,Barrett JC

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. METHODS:Re...

journal_title：Clinical chemistry

authors： Ståhlberg A,Thomsen C,Ruff D,Åman P

更新日期：2012-12-01 00:00:00

abstract：:Like any other medical technology or intervention, diagnostic tests should be thoroughly evaluated before their introduction into daily practice. Increasingly, decision makers, physicians, and other users of diagnostic tests request more than simple measures of a test's analytical or technical performance and diagnost...

journal_title：Clinical chemistry

authors： Bossuyt PM,Reitsma JB,Linnet K,Moons KG

更新日期：2012-12-01 00:00:00

abstract：BACKGROUND:Growth differentiation factor 15 (GDF15), a stress-responsive cytokine produced in cardiovascular cells under conditions of inflammation and oxidative stress, is emerging as an important prognostic marker in individuals with and without existing cardiovascular disease (CVD). We therefore examined the clinica...

journal_title：Clinical chemistry

authors： Ho JE,Mahajan A,Chen MH,Larson MG,McCabe EL,Ghorbani A,Cheng S,Johnson AD,Lindgren CM,Kempf T,Lind L,Ingelsson E,Vasan RS,Januzzi J,Wollert KC,Morris AP,Wang TJ

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND:Concomitant quantification of multiple mutant KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog) alleles may provide information in addition to that provided by standard mutation-detection procedures. We assessed the feasibility of a nanofluidic digital PCR array platform to detect and quantify KRAS...

journal_title：Clinical chemistry

authors： Azuara D,Ginesta MM,Gausachs M,Rodriguez-Moranta F,Fabregat J,Busquets J,Pelaez N,Boadas J,Galter S,Moreno V,Costa J,de Oca J,Capellá G

更新日期：2012-09-01 00:00:00

abstract：BACKGROUND:Mitochondrial diseases are clinically and genetically heterogeneous, with variable penetrance, expressivity, and differing age of onset. Disease-causing point mutations and large deletions in the mitochondrial genome often exist in a heteroplasmic state. Current molecular analyses require multiple different ...

journal_title：Clinical chemistry

authors： Zhang W,Cui H,Wong LJ

更新日期：2012-09-01 00:00:00

abstract：BACKGROUND:Hepcidin-25 reduces iron absorption by binding to the intestinal iron transporter ferroportin and causing its degradation. Currently, little is known about the basal regulation of circulating hepcidin-25. In addition, although erythropoietin administration has been reported to decrease the circulating hepcid...

journal_title：Clinical chemistry

authors： Troutt JS,Rudling M,Persson L,Ståhle L,Angelin B,Butterfield AM,Schade AE,Cao G,Konrad RJ

更新日期：2012-08-01 00:00:00

abstract：BACKGROUND:Efforts have been undertaken recently to assess the fetal genome through analysis of circulating cell-free (ccf) fetal DNA obtained from maternal plasma. Sequencing analysis of such ccf DNA has been shown to enable accurate prenatal detection of fetal aneuploidies, including trisomies of chromosomes 21, 18, ...

journal_title：Clinical chemistry

authors： Jensen TJ,Dzakula Z,Deciu C,van den Boom D,Ehrich M

更新日期：2012-07-01 00:00:00

abstract：BACKGROUND:Hyperglycosylated human chorionic gonadotropin (hCG-h) contains larger and more complex carbohydrate chains than regular human chorionic gonadotropin (hCG). hCG-h is thought to be the major form of hCG produced by testicular cancers and it has been suggested to play a key role in tumor invasion, but studies ...

journal_title：Clinical chemistry

authors： Lempiäinen A,Hotakainen K,Blomqvist C,Alfthan H,Stenman UH

更新日期：2012-07-01 00:00:00

abstract：BACKGROUND:Although lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) levels are associated with cardiovascular events, Lp-PLA(2) is physically linked to LDL cholesterol (LDL-C). Whether measures of Lp-PLA(2) mass or activity continue to predict risk after LDL-C reduction by statin therapy is uncertain. METHODS:Lp...

journal_title：Clinical chemistry

authors： Ridker PM,MacFadyen JG,Wolfert RL,Koenig W

更新日期：2012-05-01 00:00:00

abstract：BACKGROUND:Detection of circulating tumor cells (CTCs) in the peripheral blood is a rapidly developing research field with clear clinical implications for the staging and monitoring of cancer patients. Current CTC assays, including the US Food and Drug Administration-cleared CellSearch® system, typically use markers [e...

journal_title：Clinical chemistry

authors： Pantel K,Denève E,Nocca D,Coffy A,Vendrell JP,Maudelonde T,Riethdorf S,Alix-Panabières C

更新日期：2012-05-01 00:00:00

abstract：BACKGROUND:Mass spectrometric assays could potentially replace protein immunoassays in many applications. Previous studies have demonstrated the utility of liquid chromatography-multiple-reaction monitoring-mass spectrometry (LC-MRM/MS) for the quantification of proteins in biological samples, and many examples of the ...

journal_title：Clinical chemistry

authors： Hoofnagle AN,Becker JO,Oda MN,Cavigiolio G,Mayer P,Vaisar T

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND:The kidney performs a multitude of essential functions to maintain homeostasis. In clinical medicine, glomerular filtration rate (GFR) provides the best index of overall kidney function, and proteinuria adds additional information on renal and nonrenal prognosis. Several novel biomarkers of kidney injury and...

journal_title：Clinical chemistry

authors： Ferguson MA,Waikar SS

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND:Recent recognition of its broad pathophysiological importance has triggered an increased interest in 25-hydroxyvitamin D [25(OH)D]. By consequence, throughput in 25(OH)D testing has become an issue for clinical laboratories, and several automated assays for measurement of 25(OH)D are now available. The aim o...

journal_title：Clinical chemistry

authors： Heijboer AC,Blankenstein MA,Kema IP,Buijs MM

更新日期：2012-03-01 00:00:00

abstract：BACKGROUND:Technological advances have resulted in a renaissance of proteomic studies directed at finding markers of disease progression, diagnosis, or responsiveness to therapy. Renal diseases are ideally suited for such research, given that urine is an easily accessible biofluid and its protein content is derived mai...

journal_title：Clinical chemistry

authors： Konvalinka A,Scholey JW,Diamandis EP

更新日期：2012-02-01 00:00:00

abstract：BACKGROUND:We evaluated kinetic changes of high-sensitivity cardiac troponin T (hs-cTnT) in patients with acute coronary syndrome (ACS) and patients with hs-cTnT increases not due to ACS to rule in or rule out non-ST-segment elevation myocardial infarction (STEMI). METHODS:hs-cTnT was measured serially in consecutive ...

journal_title：Clinical chemistry

authors： Mueller M,Biener M,Vafaie M,Doerr S,Keller T,Blankenberg S,Katus HA,Giannitsis E

更新日期：2012-01-01 00:00:00

abstract：BACKGROUND:Plasma B-type cardiac natriuretic peptides reflect cardiac structure and function and have proven roles in assisting in the diagnosis of acute heart failure. They are independent prognostic indicators across the full spectrum of cardiovascular disease. Serial changes in plasma B-type cardiac natriuretic pept...

journal_title：Clinical chemistry

authors： Richards AM,Troughton RW

更新日期：2012-01-01 00:00:00

abstract：BACKGROUND:Although microRNAs (miRNAs) play essential roles in spermatogenesis, little is known about seminal plasma miRNAs in infertile men. We investigated the profile of seminal plasma miRNAs in infertile men to identify miRNAs that are altered in infertility; we then evaluated their diagnostic value. METHODS:Semin...

journal_title：Clinical chemistry

authors： Wang C,Yang C,Chen X,Yao B,Yang C,Zhu C,Li L,Wang J,Li X,Shao Y,Liu Y,Ji J,Zhang J,Zen K,Zhang CY,Zhang C

更新日期：2011-12-01 00:00:00

abstract：BACKGROUND:Although numerous lateral flow immunoassays (LFIAs) have been developed and widely used, inadequate analytical sensitivity and the lack of multiple protein detection applications have limited their clinical utility. We developed a new LFIA device for the simultaneous detection of high-sensitivity cardiac tro...

journal_title：Clinical chemistry

authors： Zhu J,Zou N,Zhu D,Wang J,Jin Q,Zhao J,Mao H

更新日期：2011-12-01 00:00:00

abstract：BACKGROUND:Extracellular matrix alterations are important elements in the arterial changes seen in diabetes, being associated with increased vascular stiffness and the development of cardiovascular diseases. However, no biomarkers for diabetes-related arterial changes have been defined. METHODS:Mammary artery specimen...

journal_title：Clinical chemistry

authors： Cangemi C,Skov V,Poulsen MK,Funder J,Twal WO,Gall MA,Hjortdal V,Jespersen ML,Kruse TA,Aagard J,Parving HH,Knudsen S,Høilund-Carlsen PF,Rossing P,Henriksen JE,Argraves WS,Rasmussen LM

更新日期：2011-11-01 00:00:00

abstract：BACKGROUND:Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnos...

journal_title：Clinical chemistry

authors： Kaleta EJ,Clark AE,Cherkaoui A,Wysocki VH,Ingram EL,Schrenzel J,Wolk DM

更新日期：2011-07-01 00:00:00

abstract：BACKGROUND:It is important that cardiac troponin be measured accurately with a robust method to limit false results with potentially adverse clinical outcomes. In this study, we characterized the robustness of 4 analytical platforms by measuring the outlier rate between duplicate results. METHODS:We measured cardiac t...

journal_title：Clinical chemistry

authors： Pretorius CJ,Dimeski G,O'Rourke PK,Marquart L,Tyack SA,Wilgen U,Ungerer JP

更新日期：2011-05-01 00:00:00

abstract：BACKGROUND:In 2008, the US Food and Drug Administration (FDA) issued a Guidance for Industry statement formally recognizing (during drug development) the conjoined nature of type 2 diabetes (T2D) and cardiovascular disease (CVD), which has precipitated an urgent need for panels of markers (and means of analysis) that a...

journal_title：Clinical chemistry

authors： Borges CR,Oran PE,Buddi S,Jarvis JW,Schaab MR,Rehder DS,Rogers SP,Taylor T,Nelson RW

更新日期：2011-05-01 00:00:00

abstract：BACKGROUND:Clinical proteomics presents great promise in biology and medicine because of its potential for improving our understanding of diseases at the molecular level and for detecting disease-related biomarkers for diagnosis, prognosis, and prediction of therapeutic responses. To realize its full potential to impro...

journal_title：Clinical chemistry

authors： Boja ES,Jortani SA,Ritchie J,Hoofnagle AN,Težak Ž,Mansfield E,Keller P,Rivers RC,Rahbar A,Anderson NL,Srinivas P,Rodriguez H

更新日期：2011-04-01 00:00:00

abstract：BACKGROUND:Calcitonin (CT) is a sensitive marker for evaluation of medullary thyroid cancer (MTC). However, CT measurement can vary with assay- and nonassay-dependent factors, and procalcitonin (PCT) measurement has been proposed for evaluating questionable increases in CT. METHODS:We tested 2 fully automated CT assay...

journal_title：Clinical chemistry

authors： Kratzsch J,Petzold A,Raue F,Reinhardt W,Bröcker-Preuss M,Görges R,Mann K,Karges W,Morgenthaler N,Luster M,Reiners C,Thiery J,Dralle H,Fuhrer D

更新日期：2011-03-01 00:00:00

abstract：BACKGROUND:Multiple genes that are associated with the risk of developing diabetes or the risk of diabetes complications have been identified by candidate gene analysis and genomewide scanning. These molecular markers, together with clinical data and findings from proteomics, metabolomics, pharmacogenetics, and other m...

journal_title：Clinical chemistry

authors： Malandrino N,Smith RJ

更新日期：2011-02-01 00:00:00

abstract：BACKGROUND:Diabetes mellitus has been historically divided into type 1 and type 2 diabetes, with type 1 being an autoimmune disease and type 2 being primarily a metabolic disease. CONTENT:The current diabetes classification scheme needs to be reevaluated because of the accumulating evidence of immune system involvemen...

journal_title：Clinical chemistry

authors： Brooks-Worrell B,Palmer JP

更新日期：2011-02-01 00:00:00

abstract：BACKGROUND:B-type natriuretic peptide (BNP), which is activated in heart failure (HF), is processed to an active form by corin. The corin gene is expressed in the human heart and kidney, but corin protein expression in the heart, kidney, and circulation, along with whether proBNP is processed by circulating corin, rema...

journal_title：Clinical chemistry

authors： Ichiki T,Huntley BK,Heublein DM,Sandberg SM,McKie PM,Martin FL,Jougasaki M,Burnett JC Jr

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:Massively parallel sequencing has recently been used in noninvasive prenatal diagnosis. The current costs of this technology are still relatively expensive, however, and sample throughput is still relatively low when it is used as a molecular diagnostic tool. Rather than nonselectively sequencing the genome,...

journal_title：Clinical chemistry

authors： Liao GJ,Lun FM,Zheng YW,Chan KC,Leung TY,Lau TK,Chiu RW,Lo YM

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:Plasma concentrations of small dense (sd)-LDL are associated with the prevalence of cardiovascular events. However, the special equipment and long assay times required for sd-LDL measurement have hindered its clinical application. Herein, we report development of a simple homogeneous assay for sd-LDL-cholest...

journal_title：Clinical chemistry

authors： Ito Y,Fujimura M,Ohta M,Hirano T

更新日期：2011-01-01 00:00:00